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High Performance Liquid Chromatography (HPLC) Book Course

Course Duration

5Days

Date

07 Feb 2016 – 11 Feb 2016

Venue

Singapore

Course Fee

4950

Course Description

This course offers no-nonsense, results-oriented training that will help you improve your bottom-line performance. Come prepared to discuss your analytical problems and challenges with expert chromatographers. By enrolling in this course, you will learn to master state-of-the-art techniques and equipment in HPLC with practical, hands-on experience. In the course’s hands-on laboratory sessions, you will work on a variety of experiments, and in the process, become familiar with HPLC hardware; column dimensions; mobile phase strength, flow rate, and temperature; and internal vs. external standard. By the end of the course, you will know how to develop a method for a complete unknown mixture.

Course Objective

This course offers no-nonsense, results-oriented training that will help you improve your bottom-line performance. Come prepared to discuss your analytical problems and challenges with expert chromatographers. By enrolling in this course, you will learn to master state-of-the-art techniques and equipment in HPLC with practical, hands-on experience. In the course’s hands-on laboratory sessions, you will work on a variety of experiments, and in the process, become familiar with HPLC hardware; column dimensions; mobile phase strength, flow rate, and temperature; and internal vs. external standard. By the end of the course, you will know how to develop a method for a complete unknown mixture.

Course Certificate

UMETTS Consultant certificate will be issued to all attendees completing all  of the total tuition hours of the course.

Who Should attend?

This course is designed for complete beginners to seasoned intermediates in HPLC who want practical laboratory experience. The lectures, supplemented by hands-on laboratory experiments, slides, and video tapes, provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool. No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course. Some experience in HPLC or introductory course work in organic chemistry is desirable but not necessary. Because of the amount of lab work performed by course participants, enrollment is strictly limited to 16.

Key Features of This Dynamic Cour

  • Basic instrumentation for HPLC
  • Detectors: UV/VIS, PDA, MS, Fluorescence
  • Column selection and optimization
  • Qualitative and quantitative analysis
  • Troubleshooting HPLC systems

Course Outline

HPLC Overview

  • Introduction to HPLC
  • HPLC Instrumentation Overview
  • Detector Details
  • Laboratory: HPLC Hardware

Separation Fundamentals and Mobile Phase

  • Practical HPLC Theory
  • Important HPLC Parameters
  • The Mobile Phase
  • Laboratory: Mobile Phase Strength, Flow Rate and Temperature

Separation Modes and Columns

  • Reversed Phase
  • Normal Phase
  • Ion Exchange
  • Size Exclusion
  • Laboratory: pH Effects in Reversed Phase
  • Laboratory: Column Dimensions

Quantitative Analysis and Troubleshooting

  • Qualitative and Quantitative Analysis
  • LC/MS
  • Hardware Troubleshooting
  • Separation Troubleshooting
  • Laboratory: Internal vs. External Standard
  • Hardware Troubleshooting

Gradients and Method Development

  • Gradients
  • Fundamentals of Method Development
  • Laboratory: Develop a method for a compete unknown mixture